[ PROPERTIES ]

Source: Polyclonal antibody preparation

Host: Rabbit

Purification: Antigen-specific Affinity Chromatography. Traits: Liquid

Concentration: 200µg/mL

UOM: 100µg

Applications: WB; IHC; ICC; IP.

[ IMMUNOGEN ]

Immunogen: Recombinant NOS1AP (Pro19~Ser251) expressed in E.coli. Accession No.: RPA333Mu01

[ APPLICATIONS ]

Western blotting: 0.5-2µg/mL

Immunocytochemistry in formalin fixed cells: 5-20µg/mL

Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL

Immunohistochemistry in paraffin section: 5-20µg/mL

Optimal working dilutions must be determined by end user.

[ FORMULATION ]

Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing0.05% Proclin-300, 50% glycerol.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 4oC for frequent use. Aliquot and store at -20oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation were

observed. The loss rate is less than 5% within the expiration date underappropriate storage condition.

[ IDENTIFICATION ]

A B

Figure 1. Western Blot

A. Sample: Recombinant NOS1AP, Mouse B. Mouse SerumPrimary Ab: 1µg/mL Rabbit Anti-Mouse NOS1AP AbSecond Ab: 1:2000 Dilution of HRP-Linked Guinea pigAnti-Rabbit Ab (Catalog:   IC544Rb59)